Abstract
Brucellosis is a worldwide bacterial zoonotic disease. In this study, seroprevalence of Brucella spp among domestic ruminants in Hail, Saudi Arabia was investigated and strains recovered from camels were identified based on 16S rRNA gene sequence analysis. The overall seropositivity was 10.6%, goats had a higher seroprevalence of 12.1% compared to 11.4% in sheep and 6.2% for camels as tested by RBPT. Nucleotide identity of 16S rRNA among Hail camel brucella, strains was 99.9%, while identity with Brucella strains recovered from GenBank ranged from 100 to 89.3%. To put an efficient control program in action, further research to identify the strains involved; highlights potential risks that maintain the infection in animals and/or the environment should be done.