Abstract
This study was conducted to estimate the bovine paratuberculosis seroprevalence in Khartoum and Al-Jazeera states, Sudan. The generated results showed that in Khartoum state the seroprevalence of paratuberculosis was 66.7% at the herd level and 10.2% at the individual animal level. The lowest seroprevalence (8%) was found at Kuku and the highest (18.8%) was found at El-Sealeat localities. All sera collected from El-Kadaro, Wad-Medani and El-Salama localities were found negative for Mycobacterum avium Subspecies Paratuberculosis (MAP) antibodies. Khartoum North showed the highest rate of seropositivity whereas Omdurman showed the lowest. Relationship between seroprevalence and clinical manifestation were described. The results of this study reported a widespread of bovine paratuberclosis in the Khartoum state.