Abstract
Aim: To determine the serum testosterone levels in the type 2 diabetes mellitus and its correlation with biochemical parameters of glycemic and lipid metabolism.
Methodology: A cross sectional study was conducted at Department of Biochemistry and Department of Medicine, A cross sectional study was conducted at the Department of Biochemistry, Diabetic outpatient department and Department of Medicine, Liaquat University Hospital. Male type 2 diabetics (n=100) and age matched male (n=100) were included as cases and control for study purpose. Diagnosed cases of type 2 DM, male gender and 40 - 60 years of age were included in the study protocol. Physical examination of male type 2 diabetics was performed by a consultant physician. Sera were separated from blood and stored in refrigerators at - 20 degrees C. Blood glucose, A1C, and blood lipids (cholesterol, triglycerides, LDLc and HDLc) were detected by standard laboratory methods. Serum testosterone was measured by ELISA (competitive immunoassay) assay commercial kit. Data was entered in Statistical software SPSS 21.0 version (IBM, Incorp, USA) for statistical analysis (P <= 0.05).
Results: Mean +/- SD age of male type 2 diabetics was 53.2 +/- 11.1 years compared to 54.5 +/- 10.4 years in control (P=0.056). Serum Testosterone in cases was 10.85 +/- 4.7 mmol/L compared to 13.39 +/- 3.8mmol/L in control (P=0.0001). Low testosterone level was noted in 46% male.Serum Testosterone shows inverse correlation with RBG (r= -0.31, P=0.003), A1C (r= -0.23, P=0.014), Cholesterol (r= -0.24, P=0.014), TAGs (r= --0.78, P=0.0001) and HDLc(r= -0.70, P=0.0001). Serum testosterone proved positively correlated with LDLc(r= 0.670, P=0.0001).
Conclusion: The present study finds low serum testosterone in male type 2 diabetes mellitus patients