Abstract
A new pseudoguaiane-type sesquiterpene named litopharbol (
) was isolated from the methanolic extract of the Red Sea soft coral
, along with known sesquiterpenoids alismol (
), alismorientol B (
), teuhetenone A (
), and calamusin I (
); steroid, 24-methyl-cholesta-5,24(28)-diene-3β-ol (
), alkyl glyceryl ether, chimyl alcohol (
); sphingolipid, erythro-
-dodecanoyl-docosasphinga-(4
,8
)-dienine (
); and nitrogenous bases, thymine (
) and thymidine (
). The structures were determined on the basis of nuclear magnetic resonance (NMR) spectroscopic (1D and 2D NMR data including heteronuclear single quantum coherence spectroscopy, heteronuclear multiple-bond correlation spectroscopy, and nuclear Overhauser effect spectroscopy) and mass spectrometric analyses. Compounds
were explored for antimicrobial activity and cancer cell line sensitivity tests. Compound
exhibited antibacterial activity against
with a minimum inhibition concentration of 1.8 μg/mL, whereas compound
showed significant potent cytotoxic effect against MCF-7 (breast cancer cells) with IC
4.32 μM.