Abstract
Shoot regenerations from hypocotyls and cotyledons of tomato (Lycopersicon esculentum Mill.) was inhibited by NaCl (100 and 150 mM). Shoot fresh and dry masses were also reduced. Addition of proline (100 mg dm(-3)) counteracted the inhibitory effect of NaCl. SDS-PAGE analyses of extracted proteins, revealed that in cultures grown in medium with 25 mM NaCl plus proline, extra polypeptides of M(r) 190, 58, 45 and 26 kDa accumulated. As NaCl was increased in the medium a new protein of M(r) 67 kDa also accumulated.