Abstract
Identification of the optimum application method of exogenous supports for crop plants to improve their growth under environmental stresses such as heavy metals represents key priorities for researchers worldwide. Influences of different application methods of silicon (Si; 3 mM); soil treatment, foliar spray and seed soaking on growth, chlorophyll fluorescence, photosynthetic gas exchange, cell membrane injury, osmoprotectants contents, antioxidative defense system activity, and polyamines contents and their gene expression in wheat plants grown under normal and 2 mM cadmium (Cd) stress conditions were investigated in 3-repeated pot experiment. Cd stress severely depressed growth, chlorophyll fluorescence, photosynthetic gas exchange, tissue health, water use efficiency (WUE) and Si content, and elevated osmoprotectants and Cd2+ contents, antioxidative defense system activity, and polyamines contents and their gene expression. However, Si in different application methods alleviated the Cd stress effects and significantly reduced Cd2+ and MDA contents and electrolyte leakage, significantly increased growth, chlorophyll fluorescence, photosynthetic gas exchange, WUE, membrane stability index, relative water content and Si content, and further increased proline and soluble sugars contents, antioxidative (enzymatic and non-enzymatic) defense system activity, and polyamines contents and their gene expression. Among the three methods, Si applied as soil addition was the best and most effective in alleviating the Cd stress effects.