Abstract
Silver nanoparticles (Ag NPs) were synthesized by aqueous chemical growth technique. The above mentioned synthesized materials were characterized by applying scanning electron microscope (SEM) and X-ray diffraction for confirmation of morphological analysis, compositional purity, and crystalline property and emission characteristics as well. In order to fabricate the urea biosensor (potentiometric), a solution of deionized water and chitosan was prepared having Ag NPs. The said solution was dropped on the glass fiber filter having diameter of 2 cm. A wire of copper having thickness of approximately 500 pm was used for the voltage signal to pull out from the said working nanoparticles (NPs). To improve the strength, sensitivity and the quality of the potentiometric urea biosensor, a specific functional surface of Ag NPs was attained by electrostatic restrained of an enzyme (urease) onto the chitosan-Ag (a nanobiocomposite). The potentiometric reaction was measured via electrochemical detection technique. The potentiometric urea biosensor illustrates significant sensibility at room temperature with approximate to 42 mV as per span. Furthermore, the said biosensor showed an appropriate stable response within 7 sec.