Abstract
Aim: The purpose of this study is to investigate the role of simian virus 40 (SV40) in colorectal cancer development by assessing the presence of DNA and protein product of SV40 in colorectal cancer. Materials & methods: Extracted DNA from 83 archival colorectal adenocarcinoma samples and 35 control samples of noncancerous colon tissue was analyzed for SV40 using PCR-based techniques. The expression of the protein product was assessed using immunohistochemical staining. Results: All tested tumors and control cases failed to detect SV40 DNA in PCR assays. Furthermore, immunohistochemical staining did not show any viral proteins in both cancer and control cases. Conclusion: These results indicated that there is no association between SV40 and the development of colorectal adenocarcinomas.