Abstract
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•Greener ultra-sonication methodology was used for synthesis of imine based fluorescent probe, ADD.•The ADD probe shows high selectivity and sensitivity towards ascorbic acid.•Stern Volmer quenching constant, quenching efficiency, and stoichiometry was calculated to support the existence FRET mechanism.•DFT studies have been used to confirm the binding sites of AA with ADD.•Further, it was applied for real samples analysis.
We report an optical biosensor using imine, 5-((anthrcene-9-ylmethylene) amino)-2,3dihydrophthalazine) 1–4-dione (ADD) for direct detection of ascorbic acid (AA) via FRET quenched. The ADD was successfully prepared by using simple ultra - sonication method, which was characterized by various spectroscopic techniques. The fluorescence intensity of ADD probe was drastically quenched in presence of AA, and shown excellent selectivity towards the detection of AA in presence of possible biological active interferences. A wide linear range from 0.25 to 190 µM was achieved towards the detection of AA with a LOD of 10 nM. The occurrence of FRET mechanism is due to intermolecular hydrogen bonding between ADD and AA, which was confirmed by Density Functional Theory calculations. Moreover, the biosensor was successfully applied for the detection of AA in real samples such as fruits and vegetables to demonstrate the practicability. In addition, the developed biosensor could be a simple and economically cheap platform for the detection of AA in food samples.