Abstract
Cholesterol oxidase (ChOx) has been immobilized onto sol-gel derived nano-structured cerium oxide (NS-CeO2) film deposited on indium-tin-oxide (ITO) coated glass substrate. Phase identification of sol-gel NS-CeO2 film carried out using X-ray diffraction (XRD) yields reflection peak at 29.4 degrees corresponding to (111) plane with oriented crystallite (34 nm) along c-axis normal to the substrate. Electrochemical studies reveal that NS-CeO2 provides electroactive surface for the loading of ChOx and enhances electron transfer rate in the ChOx/NS-CeO2/ITO bioelectrocle. The low value of Michaelis-Menten constant (K-m) obtained as 2.08 mM indicates enhanced ChOx affinity to cholesterol. The observed results show application of sol-gel derived NS-CeO2 for biosensing without any functionalization. (C) 2008 Elsevier B.V. All rights reserved.