Abstract
Sol-gel derived nanostructured cerium oxide (CeO2) film deposited on gold (Au) electrode has been utilized for physisorption of glucose oxidase (GOx). X-ray diffraction, atomic force microscopy, UV-visible spectroscopy, and electrochemical techniques have been used to characterize sol-gel derived CeO2/Au electrode and GOx/CeO2/Au bioelectrode. The response characteristics of the glucose bioelectrode (GOx/CeO2/Au) indicate linearity, detection limit and shelf-life as 50-400 mg/dL, 12.0 mu M, and 12 weeks, respectively. The value of apparent Michaelis-Menten constant (K-m) of GOx/CeO2/Au bioelectrode has been found to be 13.55 mu M. (c) 2008 American Institute of Physics.