Abstract
Background: Metformin is very important anti-hyperglycemic drug for non-insulin reliant diabetes mellitus. It excretes through urine. Therefore, its analysis is an important issue; especially with versatile capillary electrophoresis method. Introduction: Inexpensive solid phase micro membrane tip extraction (SPMMTE) and capillary electrophoretic (CE) methods were presented for monitoring of metformin in urine sample.
Method: Iron nano composite adsorbent was prepared by green chemistry for SPMMTE. CE experimental conditions were fused silica capillary (60 cm x 50 mu m I.D.), phosphate buffer (50 mM, 3.0 pH) as back ground electrolyte (BGE), 20 kV applied voltage, 254 nm detection, 20 seconds injection time and 27 +/- 1 degrees C working temperature.
Results: The values of electrophoretic mobility, electroosmotic mobility, migration time, number of theoretical plates and velocity were 2.71 x 10(-2) cm(2)V(-1)s(-1), 3.50 x 10(-4) cm(2)V(-1)s(-1), 10.05 minutes, 14,400 and 5.42 cm min(-1), correspondingly. The % recoveries of metformin in the laboratory prepared solution in water and urine samples were 90 and 80%.
Conclusion: It was observed that the developed SPMMTE and CE methods may be used to analyse metformin in any urine sample.