Abstract
Three species of snake-head fishes namely Channa punctatus, C. striatus and C. gachua have occurred in freshwater marshes, swamps and ditches of Indian sub-continent. The present study was to find out the species-specific marker by nuclear DNA Restrication Fragment Length Polymorphism (RFLP) using ribosomal RNA gene, probe was used for systematic analysis in fishes at the species level, species and hybrid identification. The species identification of these fishes are studied electrophoresis method.