Abstract
We investigated the occurrence and genetic diversity of
Trichoderma in the river Danube national park, a primeval, riparian forest area located south-east of Vienna (Austria) which represents one of the last cases of an original European river-floodplain landscape. Forty-six strains were isolated and identified at the species level by analysis of morphological characters, by sequence analysis of their internal transcribed spacer regions 1 and 2 (ITS1 and 2) of the rDNA cluster and – in some cases – a fragment of the translation elongation factor 1α (
tef1) gene, and RAPD-analysis. Twenty-one strains were positively identified as
T. harzianum, thirteen as
T. rossicum, four as
T.cerinum, two as
T. hamatum, and one each as
T. atroviride and
T. koningii; four strains yielded two different ITS1 and 2 as well as
tef1 sequence types, which were not alignable with any known species. Our studies show that they represent two new taxa of
Trichoderma.