Abstract
A new, simple and sensitive spectrofluorimetric method for determination of trace amount of melamine formaldehyde (
MF
) was developed. In phosphate buffer solution of pH 7.4 MF can remarkably quench the luminescence intensity of toluylene red (
NR
) at λ
em
= 590 nm due to formation of NR-MF ion associate complex. The luminescence intensity of NR-MF complex was in proportion to the concentration of MF and used as photo probe for its determination. The dynamic range for the determination of MF is 7.5–52.5 ppm with detection limit of 4.4 ppm. The method is relatively free from interferences from coexisting substances and used successfully for the determining of MF in powder and liquid forms and in wastewater produced from MF industries. The average recoveries and standard deviations of 98.3 ± 0.6, 98.1 ± 0.6 and 97.3 ± 0.5% were achieved for determination of MF in solid, liquid forms and wastewater, respectively