Abstract
Three simple and sensitive spectrophotometric methods for the determination of aminoglutethimide are described. The first method is based on the diazotization of the drug and the reaction product is quantified spectrophotometrically at 273 nm. The second and third methods involve coupling of the diazotized salt of the drug with either ethyl acetoacetate (EAA) in alkaline medium or N-(1-naphthyl)ethylenediamine hydrochloride (NED) in acid medium, to give colored reaction products measurable at 368 nm and 558 nm, respectively. The optimization of different experimental conditions is described. The proposed methods were applied successfully to the determination of amino-glutethimide in tablets with good accuracy and precision. The reliability of the developed methods were established by comparing the obtained results with those given by the official United States Pharmacopeial XXII methods.