Abstract
A simple, sensitive and precise visible spectrophotometric method is developed for the determination of pioglitazone hydrochloride in pure form, in tablets and in human urine. The method is based on the reaction of pioglitazone hydrochloride with tetrazolium blue reagent in presence of sodium hydroxide at 65 degrees C to form a pink-violet product with maximum absorbance at 518 nm. The optimum reaction conditions and reaction stoichiometry were evaluated. Linear calibration graph from 5-45 mu g/mL of pioglitazone hydrochloride was obtained with minimum detectability of 1.4 mu g/mL and a correlation coefficient of 0.9999. The relative deviation (n = 10) was 1.6. The method was applied to the determination of the drug in glustin tablets and gave good results. The method was successfully applied to the determination of pioglitazone hydrochloride in spiked human urine.