Abstract
Binuclear Cu(II) and Mn(II) newly transition metal complexes of the 5-benzylidene-3-(4-chlorophenyl)-6-oxo-5,6-dihydro-1
H
-[1,2,4]triazine-2-carbothioic acid amide (HL
1
) and 5-(3-bromo-4-methoxybenzylidene)-3-(4-chloro-phenyl)-6-oxo-5,6-dihydro-1
H
-[1,2,4]triazine-2-carbothioic acid amide (HL
2
) derived from the condensation of oxazolinone with thiosemicarbazide have been prepared. The structural elucidation was discussed upon elemental analyses and molar conductivity, as well as from mass,
1
H NMR, IR, UV-Vis spectral studies. From the analytical, spectroscopic and thermal data, the stoichiometry of the mentioned complexes was found to be 1: 2 (metal: ligand). The molar conductance data revealed that all the metal chelates are non-electrolytes and the sulphato ions existing inside of the coordination sphere. The thermal stabilities of these complexes were studied by thermogravimetric (TG-DTG/DTA) and the decomposition steps of these complexes are investigated. The kinetic thermodynamic parameters such as the energy of activation (
E
*), pre-exponential factor (
A
), activation entropy (Δ
S
*), activation enthalpy (Δ
H
*) and free energy of activation (Δ
G
*) have been calculated. Inhibitory activity against breast carcinoma cells (MCF-7 cell line), hepatocellular carcinoma cells (HePG-2 cell line) and colon carcinoma cells (HCT cell line) were tested by using different concentrations of the samples (50, 25, 12.5, 6.25, 3.125, and 1.56 μg) and cell viability (%) was determined by colorimetric method. The drug doxrubcin was used as standard.