Abstract
Melanoma is an extremely malignant skin cancer with a probability of metastasis and accountable for the mainstream skin associated mortality. In the present exploration, we described the novel usage of Stachytarpheta cayennensis-mediated copper nanoparticles (Cu-NPs) and its anticancer potential in both in vitro and in vivo model of skin cancer. The synthesis of Cu-NPs was confirmed using UV-absorbance peak values ranging from 325 to 345 nm. The size of the nanoparticles was around 90 nm, as deduced by the dynamic light scattering study. Furthermore, transmission electron microscopy (TEM) established that the morphology of the copper particles. Cytotoxicity of Cu-NPs of Stachytarpheta cayennensis illustrates the toxicity level of Stachytarpheta cayennensis. Also, the anticancer potential of Cu-NPs was evaluated in A375 cells. In experimental animals, body biochemical parameters like superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) were diminished in 7,12 dimethylbenzanthracene (DMBA) induced animals while Cu-NPs treatment raised the levels of the aforementioned enzymatic antioxidants compared to the control animals. Additionally, cytotoxicity assay, mitochondrial membrane potential (MMP), cell adhesion analysis, and the estimation of reactive oxygen species (ROS) in the presence of Cu-NPs was evaluated by standard protocols. The present study's outcomes confirm the defensive and valuable effects of copper loaded Stachytarpheta cayennensis against DMBA-induced skin melanoma, animal model.