Standards for plant synthetic biology: a common syntax for exchange of DNA parts

Nicola J Patron; Diego Orzaez; Sylvestre Marillonnet; Heribert Warzecha; Colette Matthewman; Mark Youles; Oleg Raitskin; Aymeric Leveau; Gemma Farré; Christian Rogers; Alison Smith; Julian Hibberd; Alex A R Webb; James Locke; Sebastian Schornack; Jim Ajioka; David C Baulcombe; Cyril Zipfel; Sophien Kamoun; Jonathan D G Jones; Hannah Kuhn; Silke Robatzek; H Peter Van Esse; Dale Sanders; Giles Oldroyd; Cathie Martin; Rob Field; Sarah O'Connor; Samantha Fox; Brande Wulff; Ben Miller; Andy Breakspear; Guru Radhakrishnan; Pierre-Marc Delaux; Dominique Loqué; Antonio Granell; Alain Tissier; Patrick Shih; Thomas P Brutnell; W Paul Quick; Heiko Rischer; Paul D Fraser; Asaph Aharoni; Christine Raines; Paul F South; Jean-Michel Ané; Björn R Hamberger; Jane Langdale; Jens Stougaard; Harro Bouwmeester; Michael Udvardi; James A H Murray; Vardis Ntoukakis; Patrick Schäfer; Katherine Denby; Keith J Edwards; Anne Osbourn; Jim Haseloff

doi:10.1111/nph.13532

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Standards for plant synthetic biology: a common syntax for exchange of DNA parts

Journal article

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Standards for plant synthetic biology: a common syntax for exchange of DNA parts

Nicola J Patron, Diego Orzaez, Sylvestre Marillonnet, Heribert Warzecha, Colette Matthewman, Mark Youles, Oleg Raitskin, Aymeric Leveau, Gemma Farré, Christian Rogers, …

The New phytologist, Vol.208(1), pp.13-19

10/2015

DOI: https://doi.org/10.1111/nph.13532

PMID: 26171760

Abstract

Botany

Cloning, Molecular - methods

Deoxyribonucleases, Type II Site-Specific - metabolism

DNA

Eukaryota - genetics

Genetic Engineering - methods

Genetic Engineering - standards

Plants - genetics

Plants, Genetically Modified - genetics

Plasmids

Reference Standards

Synthetic Biology - methods

Transcription, Genetic

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

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Title: Standards for plant synthetic biology: a common syntax for exchange of DNA parts
Creators - without role: Nicola J Patron - Norwich Research Park
Diego Orzaez - Instituto de Biología Molecular y Celular de Plantas
Sylvestre Marillonnet - Leibniz Institute of Plant Biochemistry
Heribert Warzecha - Technical University of Darmstadt
Colette Matthewman - Sainsbury Laboratory
Mark Youles - Sainsbury Laboratory
Oleg Raitskin - Sainsbury Laboratory
Aymeric Leveau - Norwich Research Park
Gemma Farré - Norwich Research Park
Christian Rogers - Norwich Research Park
Alison Smith - University of Cambridge
Julian Hibberd - University of Cambridge
Alex A R Webb - University of Cambridge
James Locke - Sainsbury Laboratory
Sebastian Schornack - Sainsbury Laboratory
Jim Ajioka - Sainsbury Laboratory
David C Baulcombe - Sainsbury Laboratory
Cyril Zipfel - Sainsbury Laboratory
Sophien Kamoun - Sainsbury Laboratory
Jonathan D G Jones - Sainsbury Laboratory
Hannah Kuhn - Norwich Research Park
Silke Robatzek - Sainsbury Laboratory
H Peter Van Esse - Sainsbury Laboratory
Dale Sanders - Sainsbury Laboratory
Giles Oldroyd - Sainsbury Laboratory
Cathie Martin - Norwich Research Park
Rob Field - John Innes Centre
Sarah O'Connor - Sainsbury Laboratory
Samantha Fox - Norwich Research Park
Brande Wulff - John Innes Centre
Ben Miller - John Innes Centre
Andy Breakspear - John Innes Centre
Guru Radhakrishnan - Norwich Research Park
Pierre-Marc Delaux - Norwich Research Park
Dominique Loqué - Lawrence Berkeley National Laboratory
Antonio Granell - Instituto de Biología Molecular y Celular de Plantas
Alain Tissier - Leibniz Institute of Plant Biochemistry
Patrick Shih - Lawrence Berkeley National Laboratory
Thomas P Brutnell - Donald Danforth Plant Science Center
W Paul Quick - University of Sheffield
Heiko Rischer - VTT Technical Research Centre of Finland
Paul D Fraser - Royal Holloway University of London
Asaph Aharoni - Weizmann Institute of Science
Christine Raines - University of Essex
Paul F South - Urbana University
Jean-Michel Ané - University of Wisconsin–Madison
Björn R Hamberger - University of Copenhagen
Jane Langdale - University of Oxford
Jens Stougaard - Aarhus University
Harro Bouwmeester - Wageningen University & Research
Michael Udvardi - Noble Research Institute
James A H Murray - Cardiff University
Vardis Ntoukakis - University of Warwick
Patrick Schäfer - University of Warwick
Katherine Denby - University of Warwick
Keith J Edwards - University of Bristol
Anne Osbourn - Norwich Research Park
Jim Haseloff - Sainsbury Laboratory
Publication Details: The New phytologist, Vol.208(1), pp.13-19
Grant note: BBS/E/J/00000153 / Biotechnology and Biological Sciences Research Council BBS/E/J/00000614 / Biotechnology and Biological Sciences Research Council BB/M017982/1 / Biotechnology and Biological Sciences Research Council BB/M017834/1 / Biotechnology and Biological Sciences Research Council BBS/E/J/000C0618 / Biotechnology and Biological Sciences Research Council BB/L014130/1 / Biotechnology and Biological Sciences Research Council BB/D011043/1 / Biotechnology and Biological Sciences Research Council BB/L01386X/1 / Biotechnology and Biological Sciences Research Council BB/M028860/1 / Biotechnology and Biological Sciences Research Council
Identifiers: 9944956408331
Academic Unit: King Abdullah University of Science & Technology
Language: English
Resource Type: Journal article