Abstract
Biogenesis of metal nanoparticles draws significant attention due to environment eco-friendly and sustainable methods. Locally isolated Penicillium chrysogenum MF318506 free from mycotoxins was selected as candidate for bio-synthesis of silver nanoparticles (AgNPs). The response surface methodology (RSM) of central composite design (CCD) was applied to control the biosynthesis of silver nano-size and activity depending on the pH of the reaction mixture and AgNO3 concentration. Changing conditions in the design showed different nano-sizes with different antimicrobial and cytotoxic activities. The best conditions for biosynthesis of biologically active AgNPs could be attained using the low metal concentrations (0.172 and 1.0 mM) with high pH values (8, 12&10) after 6 h incubation in dark conditions. The controlled nanoparticles were studied and characterized by UV-visible spectrophotometry and transmission electron microscopy (TEM). From this study we have concluded that the statistically controlled silver nano-size from Penicillium chrysogenum MF318506 can be utilized as a novel good candidate in biomedical research.