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Streamlined Preparation of Immobilized Candida antarctica Lipase B
Journal article   Open access  Peer reviewed

Streamlined Preparation of Immobilized Candida antarctica Lipase B

Karim Engelmark Cassimjee, Peter Hendil-Forssell, Alexey Volkov, Anne Krog, Jostein Malmo, Trond Erik V Aune, Wolfgang Knecht, Iain R Miskelly, Thomas S Moody and Maria Svedendahl Humble
ACS omega, Vol.2(12), pp.8674-8677
31/12/2017
DOI: https://doi.org/10.1021/acsomega.7b01510
PMCID: PMC6045393
PMID: 30023589

Abstract

Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L–1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.
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https://doi.org/10.1021/acsomega.7b01510View
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