Abstract
Hundung Limestone habitat, Manipur, India is an unexplored site for microbial diversity studies. Using polyphasic taxonomy, a
Streptomyces
strain, MBRL 172
T
, has been characterized. The strain was found to show highest 16S rRNA gene sequence similarity with
Streptomyces coeruleofuscus
NBRC 12757
T
(99.2 %). The DNA relatedness between MBRL 172
T
and
S. coeruleofuscus
NBRC 12757
T
, and between MBRL 172
T
and
Streptomyces nogalater
NBRC 13445
T
, were 36.8 ± 4.4 and 52.5 ± 2.7 %, respectively. Strain MBRL 172
T
was found to contain
ll
-diaminopimelic acid as the diagnostic diamino acid and glucose, mannose and xylose as the major sugars in whole cell hydrolysates. The polar lipids in the cell membrane were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones detected were MK-9(H
6
) and MK-9(H
8
). The cellular fatty acids identified were mainly saturated fatty acids: anteiso-C
15:0
, iso-C
16:0
and iso-C
15:0
. Based on differences in the biochemical and molecular characteristics from its closest relatives, the strain can be proposed to represent a novel taxon in the genus
Streptomyces
, for which the name
Streptomyces canchipurensis
is proposed, with the type strain MBRL 172
T
(=JCM 17575
T
= KCTC 29105
T
).