Abstract
Biosynthesized silver nanoparticles (Bio-SNPs) were synthesized from the marine actinobacterium strain Streptomyces catenulae M2 and characterized using a variety of techniques, including UV-vis spectrum, fourier transform infrared spectroscopy (FTIR), energy dispersive x-ray (EDX), transmission electron microscopy (TEM), dynamic light scattering (DLS), surface-enhanced Raman spectroscopy (SERS), and zeta potential. The antibacterial activity of Bio-SNPs alone and in combination with antibiotic was evaluated using a microtiter-dilution resazurin assay against multidrug-resistant (MDR) bacteria. Bio-SNPs' minimum inhibitory concentration (MIC) against bacterial strains was determined. To assess the synergistic effect of Bio-SNPs in combination with antibiotics, the Fractional Inhibitory Concentration Index (FICI) was calculated. While the safety of Bio-SNPs in biomedical applications is dependent on their use, the in vitro cytotoxicity of Bio-SNPs on normal human epithelial colon cells (NCM460) and human colorectal adenocarcinoma cells (CaCo2) were evaluated using the [3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay and cell lactate dehydrogenase (LDH) release. The presence of Bio-SNPs was revealed by UV-vis spectroscopy, which revealed a peak in the Surface Plasmon Resonance (SPR) spectrum at 439.5 nm. Bio-SNPs were spherical in shape and small in size (average 33 nm by TEM, 58.8 nm by DLS), with good stability (30 mV) and the presence of capping agents. Bio-SNPs had MIC values ranging from 2 to 64 mu g/ml against the bacteria tested. The MIC for P. aeruginosa was the lowest (2 mu g/ml). Antibiotics have been shown to have a significant synergistic effect when combined with BioSNPs against tested bacteria. Bio-SNPs exhibited dose-dependent cytotoxicity against NCM460 and CaCo2 cancer cells, with the latter exhibiting far greater toxicity than the former. NCM460 and CaCo2 cell viability decreased from 99.3 to 95.7% and 92.3 to 61.8%, respectively, whereas LDH leakage increased from 200 to 215 nmol/ml and 261 to 730 nmol/ml, respectively. The half inhibitory concentrations (IC50) for NCM460 and CaCo2 cancer cells were 79.46 and 10.41 mg/ml and 89.4 and 19.3 mg/ml, respectively. Bio-SNPs were found to be biocompatible and to have anti-inflammatory activity. Bio-SNPs are highly appealing for future nanomedicine applications due to their antibacterial and biocompatible properties and their inherent "green" and simple manufacturing.