Abstract
The continuous development of antimicrobial resistance and appearance of new diseases necessitate use of novel and effective antimicrobial molecules. This research work was planned to determine the antimicrobial and metabolite profiling of Streptomyces filamentosus strain KS17 from unexplored regions of Devbag beach, India. The Streptomyces filamentosus strain KS17 was screened and was subjected to various characterization techniques. Antagonistic activity of ethyl acetate extract was carried out and finally spectral and Gas Chromatography-Mass Spectrometry (GC-MS) analysis was done. The Streptomyces filamentosus strain KS17 displayed a strong antimicrobial activity on cross streak method. The organism produced greyish and light yellow mycelia and optimal growth was observed at 30–35 ℃ and expressed an evolutionary relationship with Streptomyces filamentosus strain Act74 (KT996121). The UV spectrum exhibited active peak at 213 nm. The ethyl acetate extract exhibited significant antimicrobial activity against bacteria and yeasts, such as S. flexneri, E. coli, K. pneumoniae, B. cereus, S. aureus, E. faecalis, B. subtilis, C. albicans and C. glabrata. The gram negative bacteria P. aeruginosa was unsusceptible. The metabolite profiling through GC-MS acquainted the presence of 30 bioactive compounds having antibacterial, anticancer properties. Pentane, 2,4-dimethyl- (40.75%), Stigmast-5-en-3-ol, oleate (17.09%), 2-methylhexacosane (4.52%), 9-Octadecenoic acid, 1,2,3-propanetriyl ester, (E,E,E)- (3.85%) and Phthalic acid, di(2-propylpentyl) ester (2.82%) were the leading compounds available in extract. These compounds are known to have strong antibacterial and antifungal activity. In conclusion, the results clearly suggest the possibility of Streptomyces filamentosus strain KS17 as optimistic candidate for the development of potential antimicrobial drugs after structure elucidation and detail characterizations of bioactive metabolite.
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•Streptomyces filamentosus strain KS17 was isolated from marine sediment samples.•The organism was identified as Streptomyces filamentosus through gene sequencing.•Streptomyces filamentosus strain KS17 showed smooth & rectiflexibiles spore chains.•The organism exhibited significant antimicrobial activity against pathogens.•The GC-MS analysis reported 30 compounds having several biological activities.