Abstract
In the present paper we prepared novel nanobioglasses with composition 40P2O5-20Na2O-10Ca(OH)2-20CaCl2-10ZnO in mol% (labeled BGZn) by using quenching melt fabrication. We investigate the thermal and microstructural
of prepared glasses by using differential thermal analysis (DTA), X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM) and Raman spectra. We were tested for its antimicrobial activity, anti-proliferative/cytotoxicity against normal and activated
splenic cells in vitro, in vivo immunologic and hypersensitivity responses, in vivo and in vitro cytotoxicity effects and in vitro effects of activated splenic cells on BGZn Results showed that BGZn (powder with nanoparticles) had antimicrobial activities
against Gramnegative and positive bacteria as well as fungi. The antimicrobial activity of nanoparticles was stronger than the powder. BGZn powder demonstrated the cytotoxic effect on normal splenic cells at high concentrations and stimulatory effect at low dilution, while nanoparticles showed
inhibitory/cytotoxic effects. BGZn powder showed no effects on activated splenic cells. In vivo test demonstrated that BGZn is an inert material which did not stimulate the immune system or caused local reactions. The products of activated splenic cells did not cause any changes in
the structure of BGZn. BGZn did not cause any acute cytotoxicity or lysis to RBCs. Finally BGZn is an inert material which did not cause immune stimulation or hepatic cytotoxicity. BGZn was not affected by lytic products of immune cells. BGZn can be considered as a safe and ideal material
for implantation inside the body of living organisms.