Abstract
Background: Uvaria narum (UN) is known to have antipyretic, antimicrobial, anti-inflammatory and antimalarial properties. The antidiabetic properties of UN remains unexplored. The current study has been aimed at understanding the antidiabetic property of UN extract on an in vitro model using 3T3-L1 cell line. Methods: Methanolic extract of UN was prepared, and its cytotoxic effect on 3T3-L1 cells was assessed. Glucose uptake and glucose transporter 4 (GLUT4) translocation in 3T3-L1 cell line on treatment with the extract was evaluated against a standard drug, metformin. alpha-glucosidase and alpha-amylase inhibition activities of the extract were also assayed with acarbose as the standard drug. Results: Treatment with UN extract had no cytotoxic effect on the cells. UN extract showed a good percentage inhibition of alpha-amylase and alpha-glucosidase activities. UN extract showed 71.31% inhibition and the control drug Acarbose exhibited 88.54% inhibition in alpha-amylase activity. Furthermore, the extract showed 79.11% inhibition when Acarbose exhibited 87.35% inhibition in alpha-glucosidase activity. IC50 values were also determined. Further, on treatment with the extract, 75.49% of 3T3-L1 cells took up glucose and 70.67% had GLUT4 expression. Conclusion: UN extract enhances glucose uptake and GLUT4 expression, inhibits alpha-amylase and alpha-glucosidase activities, thereby demonstrating the antidiabetic properties in vitro.