Abstract
Drug-induced liver injury (DILI) is considered a serious complication that may lead to liver failure. Patients may have to discontinue therapy to prevent further hepatotoxic effects of some drugs. Azathioprine (AZA) is an immune suppression drug that effectively reduces immune reaction that has been used in some immune diseases like inflammatory bowel disease (IBD), acute lymphoblastic leukemia (ALL), and organ transplants. The current study attempted to the ameliorative effects of resveratrol (RSV) and gamma-glutamylcysteine (.-GC) through antioxidative, anti-inflammation, and antiapoptotic against liver damage induced by Azathioprine. The period of the study was 28 days and included 50 male Wister Albino Rats were divided into five groups: G1 is the normal control group, G2 rats were fed AZA was administered orally (10mg/Kg body weight), G3 rats were fed resveratrol dissolved in dimethyl sulfoxide (DMSO) (8 mg/Kg body weight) were administered Intraperitoneally along with AZA administration, G4 rats were fed gammaglutamylcysteine (100 mg/Kg body weight) were administered orally along with AZA administration and G5 rats were fed resveratrol dissolved in DMSO (8 mg/Kg body weight) were administered Intraperitoneally along with AZA administration and gamma-glutamylcysteine (100 mg/Kg body weight). The results show that AZA reduces antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD). As a result, increased levels of oxidative stress reduce hepatic glutathione (GSH) and increase malondialdehyde (MDA). AZA-treated rats induce inflammation by an increase of tumor necrosis factor alpha (TNF-a). Administration of RSV or.-GC or in combination restores antioxidant enzymes, improves oxidative balance represented in restoring the depletion of GSH, and reduced lipid oxidative damage as well as reduces inflammation. Thus, the current study can be on the ameliorative effects of RSV or.-GC or in combination on liver injury in rats.