Abstract
Fourteen isolates of
Corynebacteruim pseudotuberculosis
of them 7 were isolated from sheep with Caseous Lymphadenitis "biotype 1" and 7 isolated from buffaloes with Oedematous Skin Disease "biotype 2". All isolates were identified by standard microbiological techniques and by polymerase chain reaction targeting, 16S rRNA and
phospholipase D
genes. Synergistic haemolytic titers of all isolates were assayed by plate technique. The presences of
phospholipase D
gene in supernatants of all isolates were performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis immunoblot technique by using hyperimmune serum raised in rabbit immunized with recombinant
phospholipase D
gene antigen. The concentration of
phospholipase D
gene was assayed by scanning the bound
phospholipase D
gene with specific antibodies that appeared at 31.5 kDa. Results presented that there is no correlation between titer of Synergistic haemolytic activity and the actual
phospholipase D
genes concentration in culture supernatants. Also results presented that Synergistic haemolytic activity and
phospholipase D
genes produced by biotype 2 (buffalo isolates) was generally higher than those by biotype 1(sheep isolates).