Abstract
Aseries 6-phenyl-2-(substituted methyl)-dihydropyridazinone derivatives (3a-3f) were synthesized using the interaction of 6-phenylpyridazinone with cyclic secondary amine by Mannich reaction and evaluated for antimycobacterial activity against Mycobacterium tuberculosis H37Rv strain by Microplate Alamar Blue Assay (MABA) method. All the synthesized compounds (3a-3f) were characterized by IR, H-1 NMR, and mass spectroscopy methods. The results indicated that 6-phenyl-2-(Imidazol-1-ylmethyl)-4,5-dihydro-2H-pyridazin-3-one (3b) and 6-phenyl-2-(1,2-dihydro-phenothiazin-10-ylmethyl)-4,5-dihydro-2H-pyridazin-3-one (3f) exhibited highest antimycobacterial activity. Other compounds (3a, 3c-3e) showed less significant antimycobacterial activity. The most effective compounds (3b and 3f) possessed MIC of 6.25 mu g/mL that was equal to that of reference drugs Streptomycin and Ciprofloxacin.