Abstract
In this study, hydrogelators of the L-and D-form of NapFF containing acetylene groups at the C-terminus peptides (labeled peptides 1 and 2, respectively) were synthesized and tested for their self-assembly behavior and biological activity, specifically antibacterial and mammalian cell cytotoxicity. These peptides were also tested for their biological properties. Peptides were formulated using solid-phase peptide synthesis and characterized with liquid chromatography -mass spectrometry, proton nuclear magnetic resonance, transmission electron microscopy, antibacterial assays with E. coli, and mammalian toxicity assays using HeLa and T98 cells that were obtained from ATCC. The metabolism of peptides 1 and 2 was also determined using the SMARTCyp Cytochrome P450 Metabolism Prediction Server. Carbon potion IP 8 46 247 10 On: Mon 31 Oct 2022 08:05:59 numbers 39, 37, and 26 were found tobe the most possible metabolisites for oxidation predicted using SMARTCyp. Copyright: American Scientific Publ shers Results confirmed the synthesis of the expected chemistry and naometer self-assembly of peptides 1 and 2 resembling Delivered by Ingenta that of a natural extracellular matrix. Moreover, in vitro results showed significant antibacterial activity of peptides 1 and 2 against E. coli without inducing significant mammalian cell toxicity. In this manner, results from this study highlight the promise of these peptides for a wide range of medical applications which should be further studied.