Abstract
Introduction: Antioxidants are known to prevent oxidative stress-induced damage to the biomolecules and thus, delay the onset of cancers and many age-related diseases. Therefore, the development of novel and potent antioxidants is justified. Methods: During this study, we synthesized symmetrical Bis-Schiff bases of carbohydrazide 1-27, and evaluated their in vitro antioxidative activity and cytotoxic activity. Results: Among synthesized compounds, six compounds 20 (IC50 = 12.89 +/- 0.02 mu M), 16 (IC50 = 14.32 +/- 0.43 mu M), 17 (IC50 = 18.52 +/- 0.83 mu M), 19 (IC50 = 22.84 +/- 0.62 mu M), 24 (IC50 = 35.1 +/- 0.82 mu M) and 15 (IC50 = 40.03 +/- 1.06 mu M) showed an excellent 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, better than the standard butylatedhydroxyanisole (BHA) (IC50 = 44.6 +/- 0.6 mu M). Likewise, two compounds 16 (IC50 = 4.3 +/- 1.3 mu M) and 20 (IC50 = 6.6 +/- 1.6 mu M) showed oxidative burst scavenging activity better than the standard drug ibuprofen (IC50 = 11.2 +/- 1.9 mu M). Some synthesized compounds showed good to moderate toxicity against prostate cancer (PC-3) cell lines. Conclusion: This study has identified potent antioxidants and good cytotoxic agents with the potential to further investigate.