Abstract
This work describes a simple synthesis of complexes of the type [M(C32H28N4)Cl-2], where M = Ni (II), Cu (II) and Fe (II) and a novel complex of magnetite nanoparticle (Fe3O4NP) inside (INS) tetraaza macrocyclic Schiff base ligand (C32H28N4): [Fe3O4NP-INS-(C32H28N4)], which was prepared by using a novel co-precipitation method of coordinated ferric ion (Fe3+) in the complex [Fe(C32H28N4)Cl-2] under mild conditions. The synthesized compounds were characterized and compared with a various physic-chemical techniques like: Fourier transform infrared (FT-IR), ultraviolet-visible spectroscopic techniques (UV-Vis), 1-dimensional (1D) H-1-NMR, C-13-NMR spectroscopic techniques, mass spectra, Powder X-ray diffraction (PXRD), Vibrating sample magnetometer (VSM), Scanning electron microscopy (SEM), elemental analysis and molar conductance measurements. Furthermore, the highest saturation magnetization was 26.56 emu.g(-1) obtained from [Fe3O4NP-INS-(C32H28N4)] (diameter of Fe(3)O(4)NPs20.87 nm) that prove easy separation by an external magnetic field. In vitro screening of all the compounds against different species of bacteria and fungi shows that [Fe3O4NP-INS-(C32H28N4)] is effective against the tested strains as compared to the tetraaza macrocyclic ligand and selected complexes. The cytotoxic activity of the all compounds was also examined in 3 human tumor cell lines as U87, MDA-MB-231 and LS-174. The complex [Fe3O4NP-INS-(C32H28N4)] shows moderate and strong cytotoxic activity against brain cancer, colon cancer and breast cancer (U87, MDA-MB-231 and LS-174 respectively), without showing cytotoxicity towards peripheral blood mononucleocyte (PBMC) cells.