Abstract
A series of 1,2,4-triazole hydrazones 1-25 has been synthesized and characterized using different spectroscopic techniques including FT-IR, H-1-NMR, and ESI MS spectrometry. The synthetic derivatives were evaluated for their beta-glucuronidase enzyme inhibition properties. Among them, 17 compounds demonstrated potential inhibitory activity towards beta-glucuronidase with IC50 values ranging between 2.50 and 53.70 A mu M. Compounds 1 having IC50 = 2.50 +/- 0.01 A mu M was found to be the most active compound of the series and showed remarkable activity and found to be far more potent than the standard d-saccharic acid 1,4-lactone (IC50 = 48.4 +/- 1.25 A mu M). Furthermore, the possible binding interaction of active compounds was explored by in silico studies. These compounds can be used for anti-diabetic drug development process.