Abstract
Nickel(II) complex Ni(L)(2).H2O (1) of diimine Schiff base N,N-bis-(sa1icylaldehyde)-o-phenylenediamine (SalophH(2)) ligand was obtained and confirmed by analytical and spectroscopic techniques. The structural X-ray analysis clearly indicated that 1 possesses distorted square planar geometry around the Ni(II) atoms. In vitro binding of 1 with calf thymus (CT-DNA) performed via a series of biophysical measurements, which suggested that 1 binds with DNA via surface electrostatic and minor groove modes with binding constant 3.82 +/- 0.15 x 10(4) M-1. The hydrogen bonding interaction of terminal ethoxy and C=N groups of 1 with the base pairs of DNA, leads to partial destabilization of the DNA double helix. Similarly, complex has the ability to quench the intrinsic fluorescence of HSA through static quenching mode with moderate binding affinity (K=2.15 +/- 0.13 x 10(4) M-1) as compared with DNA. Furthermore, docking analysis with HSA (1AO6), DNA (1BNA) and G-tetrad were also employed to analyze the ability of the complex to interact with various macromolecular target. (C) 2022 Elsevier B.V. All rights reserved.