Abstract
Hydroalcoholic extracts have been prepared from Linum usitatissimum with maceration method, which was later examined for its antimicrobial activity in the presence of CuS nanoparticles by broth macrodilution and agar disc diffusion, and the determination of minimal inhibitory concentration of antibacterial agent required to inhibit growth of a pathogen, i.e. minimal inhibitory concentration and the lowest concentration of an antibacterial agent required to kill a particular bacterium, i.e. minimal bactericidal concentration were well elucidated and evaluated for these materials. The superposition of metal nanoparticles with the L. usitatissimum extracts was found to be effective in the eradication of the bacterial infections, and proved to be a good alternative of antibiotics. Antioxidant content of the extracts was also determined and demonstrated the highest antioxidant activities associated with the shoot of L. usitatissimum (Total phenolic content 1: 128.24 +/- 1.127-mg gallic acid equivalents/g of dried extract, DPPH: 30.57 +/- 0.4% inhibition, Ferric reducing antioxidant power: 957.8 +/- 3.81 mol Fe(II)/mg of dried extract and Total Flavonoid Content: 2: 95.04 +/- 0.53-mg rutin equivalents (RuE)/g of dried extract).