Abstract
Green synthesis of silver nanoparticles (AgNPs) has consistently revolutionised the field of nanotechnology, and bio-based AgNPs have emerged as efficient therapeutic tools in biomedical science. Synthesis and characterization of silver nanoparticles from Brassica nigra (BN) and evaluation of antifungal, anticancer antibacterial, and antioxidant activities were investigated in this study. The characteristics of BN-AgNPs was studied using various spectroscopic techniques measuring ultraviolet-visible (UV-Vis) spectra, determination of particle size, zeta potential, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Elemental composition was determined using energy-dispersive spectroscopy (EDX). Formation of AgNPs was confirmed by UV-Vis spectroscopy. Cytotoxic activity was determined at different nanoparticle concentrations on the MCF-7 cell line with the maximum cytotoxic effect observed at 100 mu g/ml. BN-AgNPs also showed strong antifungal and antimicrobial activities along with antioxidant properties. The green synthesis of nanoparticles is comparatively simple, eco-friendly and safer than conventional methods and the observed anticancer activity in the current report is suggestive of the biogenic AgNPs as therapeutic agent for cancer therapy. (C) All Rights Reserved