Abstract
This paper describes a facile protocol, efficient, and environmentally benign for the synthesis a series of barbiturate acid substituted at C5 position 3a-o. The desired compounds subjected in vitro for different set of bioassays including against anti-oxidant ( DPPH and super oxide scavenger assays), anti-cancer, alpha-glucosidase and beta-glucuronidase inhibitions. Compound 3m (IC50 = 22.9 +/- 0.5 mu M) found to be potent alpha-glucosidase enzyme inhibitors and showed more activity than standard acarbose (IC50 = 841 +/- 1.73 mu M). Compound 3f (IC50 = 86.9 +/- 4.33 mu M) found to be moderate beta-Glucuronidase enzyme inhibitors and showed activity comparatively less than the standard D-saccharic acid 1,4-lactone ( IC50 = 45.75 +/- 2.16 mu M). Furthermore, in sillico investigation was carried out to investigate bonding mode of barbiturate acid derivatives. (C) 2016 Elsevier Inc. All rights reserved.