Abstract
Axillary buds from Rosa damascena trigintipetala Dieck (Al-Taif rose) Plant proliferated in vitro were encapsulated in different concentrations and combinations of sodium alginate (SA), calcium chloride (CaCl2) and alginate bead composition, to investigate their effects on the conversion rates of synseed before and after preservation duration at 4 degrees C. The early capsules break after 5 days and whole capsules germinated 100% after 10 days from culture, were noticed on control (non capsulated nodal segments) and the beads ZPC (zero period of conservation) that formed by 3% of SA and 75 mM of CaCl2. A very delay in capsule break (12 and 14 days) and capsule germination percentage 100% after 25 and 30 days were noticed on synseed polymerized by 5% of SA, 75 and 100 mM of CaCl2. After 2 weeks from preservation, axillary buds encapsulated in 3% and 4% of SA and 75 mM of CaCl2, showed capsules break after 14 days of culture, with germinated capsules percentage 80 and 50%, respectively. However, 5% of SA, 75 and 100 mM of CaCl2 exhibited no wall capsule break and germination capsule. The highest percentage 90 and 80% of average number of germinated artificial seeds ZPC were resulted from 3% of SA fortified with 1/2 strength Murachige and Skoog medium (MS)+ 0.5% of sucrose and control (half-strength MS free sucrose and gibbrelic acid). After four and eight weeks from conservation, alginate beads enriched with half-strength MS+0.5 % of sucrose showed the maximum value 70 and 60% after four and eight weeks from storage. According to our results, synthetic seeds could be used for in vitro preservation of Al-Taif rose (Rosa damascena trigintipetala Dieck) plant.