Abstract
The current development was focused on environmentally friendly and cost-effective synthesis of silver nanoparticles (AgNPs). This investigation aims to synthesize AgNPs using Anchusa arvensis aqueous plant extract. The characterization was confirmed by means of different techniques
including, X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FT-IR) Spectroscopy, and UV-visible analysis. The crystalline morphology of AgNPs is demonstrated by X-ray diffraction, with mean crystallite diameter ranges from 20-25 nm. The spherical
geometry of particles was revealed via SEM images. The FT-IR Spectroscopy confirmed that, aqueous extract of plant contains essentials phytoconstituents such as, O-H stretching vibration of carboxylic acid, C-H is the stretching vibration of alkane, C-O indicate the stretching
vibration of alcohol, C=C corresponds to stretching vibration of aromatic compounds, which were responsible for the formation of AgNPs. The synthesized nanoparticles were stable at temperatures up to 50 °C, as well as in neutral and basic pH, but were unstable at acidic pH. The synthesized
AgNPs had promising antileishmanial and THP-1 Cell line cytotoxic activity against Leishmanial promastigotes, with an averaged mortality (%) of 85.3±0.7% at IC50 values of 35.3±1.81 μg/mL and 65.3±0.7% with IC50 values of 45.3±1.81
μg/mL, respectively. The brine shrimp lethality of synthesized nanoparticles showed that the concentration greater than 200 μg/mL was biologically compatible.