Targeted gene deletions in C. elegans using transposon excision

Christian Frokjaer-Jensen; M. Wayne Davis; Gunther Hollopeter; Jon Taylor; Todd W. Harris; Paola Nix; Rachel Lofgren; Michael Prestgard-Duke; Michael Bastiani; Donald G. Moerman; Erik M. Jorgensen

doi:10.1038/NMETH.1454

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Targeted gene deletions in C. elegans using transposon excision

Journal article

Peer reviewed

Targeted gene deletions in C. elegans using transposon excision

Christian Frokjaer-Jensen, M. Wayne Davis, Gunther Hollopeter, Jon Taylor, Todd W. Harris, Paola Nix, Rachel Lofgren, Michael Prestgard-Duke, Michael Bastiani, Donald G. Moerman, …

Nature methods, Vol.7(6), pp.451-U57

01/06/2010

DOI: https://doi.org/10.1038/NMETH.1454

PMCID: PMC2878396

PMID: 20418868

Abstract

Biochemical Research Methods

Biochemistry & Molecular Biology

Life Sciences & Biomedicine

Science & Technology

We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.

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Title: Targeted gene deletions in C. elegans using transposon excision
Creators - without role: Christian Frokjaer-Jensen - University of Utah
M. Wayne Davis - University of Utah
Gunther Hollopeter - Howard Hughes Medical Institute
Jon Taylor - University of British Columbia
Todd W. Harris - Hi Line Informat, Livingston, MT USA
Paola Nix - University of Utah
Rachel Lofgren - Howard Hughes Medical Institute
Michael Prestgard-Duke - Howard Hughes Medical Institute
Michael Bastiani - University of Utah
Donald G. Moerman - University of British Columbia
Erik M. Jorgensen - Howard Hughes Medical Institute
Publication Details: Nature methods, Vol.7(6), pp.451-U57
Publisher: Springer Nature
Number of pages: 5
Grant note: Lundbeck Foundation; Lundbeckfonden McKnight Grant Genome British Columbia R37NS034307 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) Howard Hughes Medical Institute Genome Canada
Identifiers: 9945007608331
Academic Unit: King Abdullah University of Science & Technology
Language: English
Resource Type: Journal article