Abstract
•The removed of melamine (MA) was mainly performed by activated sludge adsorption instead of biodegradation.•High concentration of MA could not easily removal and had adverse impacts on biological wastewater treatment.•MA inhibited the enzyme activities of NOR, NR, NIR and PPX, which were closely relevant to nitrogen and phosphorus removal.•High MA concentrations promoted the metabolism of glycogen, thereby providing the advantage for the growth of GAOs.
Melamine (MA) is a significant raw material for industry and home furnishing, and an intermediate for pharmacy. However it is also a hazardous material when being added to food as a protein substitute due to the high nitrogen content. In this study, the behavior of MA in activated sludge was investigated. Experiments showed that MA was removed during biological wastewater treatment process, and the removal was mainly achieved by activated sludge adsorption instead of biodegradation. Low levels of MA (0.001–0.10mg/L) in wastewater had negligible influence on the performance of activated sludge, but high levels of MA deteriorated biological nitrogen and phosphorus removal. The presence of MA (1.00 and 5.00mg/L) decreased total nitrogen removal efficiency from 94.15% to 79.47% and 68.04%, respectively. The corresponding concentration of effluent phosphorus increased from 0.11 to 1.45 and 2.06mg/L, respectively. It was also observed that MA inhibited the enzyme activities of nitrite oxidoreductase, nitrate reductase, nitrite reductase and exopolyphosphatase, which were closely relevant to nitrogen and phosphorus removal. Further investigation showed that the presence of high MA concentrations promoted the consumption and synthesis of glycogen, thereby providing the advantage for the growth of glycogen accumulating organisms.