Abstract
- OBJECTIVE: The aim of the study was to determine the bacterial-adherence to the experimental pellicle pretreated with commer-cially available oral-rinse/photosensitize r (mim-icking use of oral-rinse/photosensitize r before meals). MATERIALS AND METHODS: An artificial mouth (NAM) system was used for the devel-opment of single-species biofilm (Actinomyces viscosus, Streptococcus mitis and Streptococ-cus sanguinis respectively). Two commercially available oral-rinses containing active ingredi-ents [Essential oils (EO) and Chlorhexidine glu-conate (CHX) were used. Curcumin photosensi-tize r (PS) was used as a photosensitizer against the microbes. For the adherence study, the ex-perimental pellicle on the beads (in the capillary tubes of the NAM system) was pretreated with the oral-rinse and photosensitizer before the in-oculation of bacteria; this would resemble the use of rinse/photosensitize r before meal. The bacterial population of the biofilm was deter-mined using serial dilution assay and expressed as colony forming unit per ml. Deionized dis-tilled water was used in place of oral-rinse/ pho-tosensitize r and served as a negative-control. For the qualitative study, bacterial population viewing was carried out using Scanning Elec-tron Microscope (SEM). RESULTS: It was observed that on treatment with the oral-rinses the bacterial population of S.mitis, S.sanguinis and A.viscosus (adherence) was significantly reduced where the reduction was less for EO-based oral-rinse compared with that of CHX and curcumin PS in the following se-quence EO0.05). CONCLUSIONS: From the results, it appears that curcumin photosensitizer and oral-rinses contain CHX to be preferably used before-meal and EO after-meal.