The need for transparency and good practices in the qPCR literature

Stephen A. Bustin; Vladimir Benes; Jeremy Garson; Jan Hellemans; Jim Huggett; Mikael Kubista; Reinhold Mueller; Tania Nolan; Michael W. Pfaffl; Gregory Shipley; Carl T. Wittwer; Peter Schjerling; Philip J. Day; Monica Abreu; Begona Aguado; Jean-Francois Beaulieu; Anneleen Beckers; Sara Bogaert; John A. Browne; Fernando Carrasco-Ramiro; Liesbeth Ceelen; Kate Ciborowski; Pieter Cornillie; Stephanie Coulon; Ann Cuypers; Sara De Brouwer; Leentje De Ceuninck; Jurgen De Craene; Helene De Naeyer; Ward De Spiegelaere; Kato Deckers; Annelies Dheedene; Kaat Durinck; Margarida Ferreira-Teixeira; Annelies Fieuw; Jack M. Gallup; Sandra Gonzalo-Flores; Karen Goossens; Femke Heindryckx; Elizabeth Herring; Hans Hoenicka; Laura Icardi; Rolf Jaggi; Farzad Javad; Michael Karampelias; Frederick Kibenge; Molly Kibenge; Candy Kumps; Irina Lambertz; Tim Lammens; Amelia Markey; Peter Messiaen; Evelien Mets; Sofia Morais; Alberto Mudarra-Rubio; Justine Nakiwala; Hilde Nelis; Pal A. Olsvik; Claudina Perez-Novo; Michelle Plusquin; Tony Remans; Ali Rihani; Paulo Rodrigues-Santos; Pieter Rondou; Rebecca Sanders; Katharina Schmidt-Bleek; Kerstin Skovgaard; Karen Smeets; Laura Tabera; Stefan Toegel; Tim Van Acker; Wim Van den Broeck; Joni Van der Meulen; Mireille Van Gele; Gert Van Peer; Mario Van Poucke; Nadine Van Roy; Sarah Vergult; Joris Wauman; Marina Tshuikina-Wiklander; Erik Willems; Sara Zaccara; Fjoralba Zeka; Jo Vandesompele

doi:10.1038/nmeth.2697

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The need for transparency and good practices in the qPCR literature

Journal article

Peer reviewed

The need for transparency and good practices in the qPCR literature

Stephen A. Bustin, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W. Pfaffl, Gregory Shipley, …

Nature methods, Vol.10(11), pp.1063-1067

01/11/2013

DOI: https://doi.org/10.1038/nmeth.2697

PMID: 24173381

Abstract

Biochemical Research Methods

Biochemistry & Molecular Biology

Life Sciences & Biomedicine

Science & Technology

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

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Title: The need for transparency and good practices in the qPCR literature
Creators - without role: Stephen A. Bustin - Anglia Ruskin University
Vladimir Benes - European Molecular Biology Organization
Jeremy Garson - University College London
Jan Hellemans - Biogazelle NV, Zwijnaarde, Belgium
Jim Huggett - LGC
Mikael Kubista - Tataa Biocenter
Reinhold Mueller - ABS Consulting
Tania Nolan - Sigma
Michael W. Pfaffl - Technical University of Munich
Gregory Shipley - Shipley Consulting, Austin, TX USA
Carl T. Wittwer - University of Utah
Peter Schjerling - Bispebjerg Hospital
Philip J. Day - University of Manchester
Monica Abreu - University of Coimbra
Begona Aguado - Centro de Biología Molecular Severo Ochoa
Jean-Francois Beaulieu - Université de Sherbrooke
Anneleen Beckers - Ghent University Hospital
Sara Bogaert - Ghent University Hospital
John A. Browne - University College Dublin
Fernando Carrasco-Ramiro - Centro de Biología Molecular Severo Ochoa
Liesbeth Ceelen - Helicon Foundation
Kate Ciborowski - University of Bristol
Pieter Cornillie - Ghent University
Stephanie Coulon - Ghent University Hospital
Ann Cuypers - Hasselt University
Sara De Brouwer - Ghent University Hospital
Leentje De Ceuninck - Ghent University
Jurgen De Craene - Ghent University
Helene De Naeyer - Ghent University
Ward De Spiegelaere - Ghent University
Kato Deckers - KU Leuven
Annelies Dheedene - Ghent University Hospital
Kaat Durinck - Ghent University Hospital
Margarida Ferreira-Teixeira - University of Coimbra
Annelies Fieuw - Ghent University Hospital
Jack M. Gallup - Iowa State University
Sandra Gonzalo-Flores - Centro de Biología Molecular Severo Ochoa
Karen Goossens - Ghent University
Femke Heindryckx - Uppsala University
Elizabeth Herring - Université de Sherbrooke
Hans Hoenicka - Thunen Inst Forest Genet, Grosshansdorf, Germany
Laura Icardi - Ghent University
Rolf Jaggi - University of Bern
Farzad Javad - University of Manchester
Michael Karampelias - VIB-UGent Center for Plant Systems Biology
Frederick Kibenge - University of Prince Edward Island
Molly Kibenge - Univ Prince Edward Isl, Atlantic Vet Coll, Charlottetown, PE C1A 4P3, Canada
Candy Kumps - Ghent University Hospital
Irina Lambertz - Ghent University Hospital
Tim Lammens - Ghent University Hospital
Amelia Markey - University of Manchester
Peter Messiaen - Ghent University Hospital
Evelien Mets - Ghent University Hospital
Sofia Morais - Institute for Research and Technology in Food and Agriculture
Alberto Mudarra-Rubio - Centro de Biología Molecular Severo Ochoa
Justine Nakiwala - Instituut voor Tropische Geneeskunde
Hilde Nelis - Ghent University
Pal A. Olsvik - Natl Inst Nutr & Seafood Res, Bergen, Norway
Claudina Perez-Novo - Ghent University Hospital
Michelle Plusquin - Hasselt University
Tony Remans - Hasselt University
Ali Rihani - Ghent University Hospital
Paulo Rodrigues-Santos - University of Coimbra
Pieter Rondou - Ghent University Hospital
Rebecca Sanders - LGC
Katharina Schmidt-Bleek - Berlin Institute of Health at Charité - Universitätsmedizin Berlin
Kerstin Skovgaard - Technical University of Denmark
Karen Smeets - Hasselt University
Laura Tabera - Centro de Biología Molecular Severo Ochoa
Stefan Toegel - Medical University of Vienna
Tim Van Acker - Ghent University
Wim Van den Broeck - Ghent University
Joni Van der Meulen - Ghent University Hospital
Mireille Van Gele - Ghent University Hospital
Gert Van Peer - Ghent University Hospital
Mario Van Poucke - Ghent University
Nadine Van Roy - Ghent University Hospital
Sarah Vergult - Ghent University Hospital
Joris Wauman - Ghent University
Marina Tshuikina-Wiklander - Sigma Aldrich Sweden AB, Stockholm, Sweden
Erik Willems - Sanford Burnham Prebys Medical Discovery Institute
Sara Zaccara - Ctr Integrat Biol CIBIO, Lab Transcript Networks, Trento, Italy
Fjoralba Zeka - Ghent University Hospital
Jo Vandesompele - Ghent University Hospital
Publication Details: Nature methods, Vol.10(11), pp.1063-1067
Publisher: Springer Nature
Number of pages: 5
Identifiers: 9943719708331
Academic Unit: King Abdullah University of Science & Technology
Language: English
Resource Type: Journal article