Abstract
1.
1. Phosphofructokinase from camel liver was purified to homogeneity more than 3600-fold, and the yield of the preparation was 46%.
2.
2. The sodium dodecyl sulphate-treated purified enzyme migrated as a single band in 10% polyacrylamide gel.
3.
3. The enzyme is a tetramer, with a monomer
M
r 90,000.
4.
4. The regulatory properties of the purified enzyme from camel liver were studied at pH 7.0.
5.
5. The enzyme displayed cooperativity with respect to fructose 6-phosphate and was inhibited by high concentrations of ATP.
6.
6. The enzyme was also inhibited by citrate, phosphocreatine and 2,3-bisphosphoglycerate.
7.
7. On the other hand, ADP, AMP, glucose 1,6-bisphosphate and fructose 2,6-bisphosphate were all found to be strong activators for camel liver phosphofructokinase.