Abstract
This study full filed in enhancement of catalytic, thermodynamics and storage stability of Altemaria tenuissima KM651985 laccase by conjugation to sodium periodate oxidized starch. The starch conjugated A. tenuissima KM651985 laccase was active over a wide range of temperatures and pHs with the highest activity at 60 degrees C and 4, respectively. The thermal stability of conjugated A. tenuissima KM651985 laccase was indicated by, high TIp values (half life) 1076.16, 382.42 and 19123 min at 50, 60 and 70 degrees C, respectively, low K-d (denaturation rate constant) 6.44 x 10(-4), 18.13 x 10(-4) and 36.25 x 10(-4) min(-1) at the same temperatures, high D-values (decimal reduction time) 3575.56, 1270.61 and 635.38 min at the same temperatures. Also, the thermal stability of conjugated A tenuissima KM651985 laccase was emphasized by high Delta H-d (enthalpy), high Delta G(d) (free energy) and low Delta S-d (entropy). The conjugated A tenuissima KM651985 lactase showed high effectiveness in dyes decolorization of Remazol Brilliant Blue R (RBBR) and Malachite Green (MG). Moreover, the addition of conjugated A. tenuissima KM651985 laccase with hemicellulolytic enzymes cocktail improved the saccharification of corn cobs, rice straw, corn cobs leaves and water hyacinth with the highest reducing sugar production 847.44 +/- 19.17 mg from corn cops. (C) 2018 Elsevier B.V. All rights reserved.