Abstract
Chronic diabetes with uncontrolled hyperglycemia is associated with impaired wound healing due to increased oxidative stress and low nitric oxide (NO) level in wound tissue. Nanotechnology enables synthesizing nanoparticle with efficient drug delivery. The aim of the present study was to evaluate bandage formulated with nitric oxide donor nanoparticles (NONPs) on wound healing in STZ-induced DM in mice. In the present study nitric NONPs was prepared as powder size ranging from 19-28 nm and mixed with vaseline to form cream bandaged for topical application. Two excisional wounds were made on the dorsa of mice. The present results demonstrated significantly enhanced healing after 7 and 14 days from induction of the wound. Compared to diabetic mice, wound tissue showed increased levels of NO and GSH, upregulated activities of SOD and CAT and decreased MDA content. Topical application of NONPs bandage improved wound vascularization evidenced histologically and by significantly increased vascular endothelial growth factor (VEGF) and transforming growth factor beta 1 (TGF-B1) in serum. The present results emphasize the potential of a new nitric oxide platform for wound healing therapy.