Abstract
•Larvicidal activity of ethanolic extracts of marine Champia parvula (Ex-Cp) against the dengue mosquito vector Ae. aegypti.•Sub-lethal dosage of Ex-Cp significantly alters the physiological as well as enzyme activity of carboxylesterase (α and β), GST and CYP450.•Ex-Cp displayed significant repellent activity (97%) with maximum level of protection time (210 minutes) at the lethal dosage of 100 ppm.•Non-target toxicity of Ex-Cp against the beneficial aquatic mosquito predators displayed less toxicity.
Larval toxicity of ethanolic extract of C. parvula (Ex-Cp) was prominent in the second and the third instars at the maximum lethal dosage of 100 ppm with 98 and 97 % mortality rate respectively. The LC50 and LC90 was displayed at 43 ppm and 88 ppm dosage respectively. Correspondingly, the sub-lethal dosage (65 ppm) of Ex-Cp significantly alters the carboxylesterase (α and β), GST and CYP450 enzyme level in both III and IV instar larvae in dose-dependent manner. Similarly, the Ex-Cp displayed significant repellent activity (97 %) with a maximum level of protection time (210 min). Photomicrography assay of Ex-Cp (65 ppm) were toxic to dengue larvae as compared to control. The non-target toxicity of Ex-Cp against the beneficial mosquito predators displayed less toxicity at the maximum dosage of 600 ppm as compared to Temephos. Thus the present research delivers the target and non-target toxicity of red algae C. parvula against the dengue mosquito vector.