Abstract
To further investigate the promoting effect of soybean protein and peptides on Lacticaseibacillus rhamnosus Lra05 (L. rhamnosus Lra05), the overall changes of gene expression of it was investigated through transcriptome analysis treated with dPEP and dSPI The results showed that there were 890, 113, and 1073 differentially expressed genes (DEGs) in dPEP/MRS, dSPI/MRS and dPEP/dSPI comparison, respectively. Besides, we found dPEP could promote the expression of acc and fab family genes related to fatty acid biosynthesis, but dSPI played the opposite role. Moreover, dPEP stimulated genes clustered in pur family and ribosome protein, responsible for purine and protein synthesis. Interestingly, dPEP suppressed genes regulating peptides and amino acids trans-portation and dSPI specially inhibited the pyr family genes involved in pyrimidine metabolism. These data revealed the knowledge about the metabolic pathways affected by dPEP and dSPI and provide a preliminary understanding of the relationship between soybean proteins and peptides and L. rhamnosus Lra05.