Abstract
A rapid high-performance liquid chromatographic procedure for separation of the triacylglycerols (TG) of animal fats using refractive index detection is described. A LiChrospher-100 RP-18 (5 μm) column was used for the TG-profiling of pork, beef, mutton, chicken and turkey fats. Detection of pork fat in processed foods and lard in fat-admixtures is also discussed. TG-separation and checking genuinity and adulteration was achieved isocratically in ∼ 15 min. by using CH
3
CN/CH
2
Cl
2
(58:42, v/v) at ambient temperature.